An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each. Thank you!. Multimode microplate reader equipped with latest detections like fluorescence, absorbance etc. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). 2013): the specific activity of both enzymes was ~1. Maximum signal with lowest background. † Cells in the paper scaffolds and collected in the well plates were also imaged with an Olympus IX-70 fluorescence microscope equipped with. reader. 14. edu to schedule training. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 9000-0756. Perkin Elmer Victor X4 Multimode Plate Microplate Reader 2030-0040 with PC. IC 50 values were determined on a multimode SpectraMax M5 plate reader using 96-well plates (Thermo Fisher Scientific) by monitoring absorbance at 412 nm taking measurements every 30 s for 20 min. All kinetic reads were taken at 25 °C in nonbinding all black clear-bottomed Greiner 96-well plates covered with optically clear films and stirred for 10 s prior to each reading. 2 MΩ cm. Wells with an OD 600[0. 305 reference level saturation. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Imagine a world where everyone smiles®. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. About. DH5α and BL21 (DE3) chemically competent E. 3. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 1. 8K members Since 2000 M5Board is the best forum community for information on the BMW M5 E60 (V-10), E39 (V-8), E34. . 5 mmol/L Li 2 SO 4 for 48 h. M-5 The M-5 multitronic unit, or the M-5 computer, was an advanced multitronic computer system and prototype created by Doctor Richard Daystrom during. Spend a little more and you can get an FTDI-based USB cable. 9. Published Dec 16, 2020 | Updated Dec 16, 2020. RUO. How to export results files from the plate reader into Excel. Data were expressed as % control =. 2. Fluorescence was measured with the SpectraMax M5 plate reader with the same excitation and emission wavelengths as the chemical denaturation assay. We use Spectramax M5 microplate reader in our lab. Primary human airway. HeLa cells were seeded in a 6-well plate with standard DMEMfluorescence intensity in the supernatant was measured in a SpectraMax M5 plate - reader (Molecular Devices) at 485 nm excitation and 535 nm emission wavelengths. Microplate Reader Shelf. 3. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Plate Analysis 9. Plate stacker and robot integration The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Quantitative PCR RNA was collected in duplicate from 1 × 10 6 cells lysed in TRIzol Reagent (Invitrogen, 15596018) after. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. 25 pM (0. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. After this time period, the plate was read for fluorescence intensity at ex/em wavelengths of 532/590 nm, on a SpectraMax M5 plate reader. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. Information on how to reserve the plate readerSunnyvale, California -- October 5, 2017. 5 h (during the lag phase), 7 h (during the exponential phase), and 24 h (during the plateau phase) for analysis of the fermentation. Plate Reader with 5 modes of detection, UV-Vis (Abs), Fluorescence (FI) , Lumi, TRF, F Polarization, with current validations, fully inspectedOpen the Gen 5 software, 2. After the plate is read, data will appear in the plate section: 11. Plate Shaking. Application Area: Multi mode plate reader. The Back Panel Figure 1. Typically the system can detect absorbance, luminescence, fluorescence,. Purified fluorescent protein was diluted 15-fold into 0. 5. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 2M posts 124. SA-β-gal assay. Top reading usually provides better signal-to-noise ratios for solution-based assays such as DNA quantification or protein quantification. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. A solution for low volume applications. Protocol for Qubit DNA BR Assay. AC16 cells were treated with control, 5 mmol/L LiCl or 2. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Three kits covering a wide range of dsDNA concentrations. 1. 17. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax. 4. One hundred microliters of the supernatant was then transferred into a 96-well plate and read in a SpectraMax M5 plate reader (Molecular Devices) at A570. Optimized for fluorescence microplate readers. Four-parameter curve-fitting software (Softmax Pro) was used to convert optical density values to denosumab concentrations. 5. Detection Modes. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. were read using SpectraMax M5 plate reader (Molecular Devices, San Jose, CA, USA) with excitation of 380 nm and emission of 460 nm. PURPOSE. The fluorescent intensity of the cells reflecting the LIP was quantified by measuring the fluorescent signal at excitation wavelength (λex) of 543 nm and emission wavelength (λem) of 580 nm as per the manufacturer recommendation using a SpectraMax M5 plate reader (Molecular Devices) and normalized to the cell count. Table of Contents. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 1 M glycine-phosphate-citrate buffers with 0. ThT fluorescence was measured at 444 and. 0 μM), and this response was consistent in both plate reader assays and on chip (Supplementary Figure 5d. In the closed conformation, the regulatory SH3 and SH2 domains pack against the back of the kinase domain, providing allosteric control of kinase activity. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. The plate reader assay was then repeated in SC-URA media with and without. The concentration of MMP-9 was measured using R&D System’s ELISA Kit (Minneapolis, MN) on a Spectramax M5 plate reader equipped with SoftmaxPro v5. The absorbance was measured using Molecular Devices Spectramax M5 plate reader with the software Softmaxpro at 570 and 650 nm. There is currently no charge for using this plate reader. Plates were incubated at 37°C for 6 hours and then equilibrated to room temperature for 30 min. With optimized reagents, option to operate the readers. Molecular Devices M5 plate readers; Multiple liquid bulk dispensers (Wellmate, Multidrop Combi) Price List. Turn on Plate Reader and wait for it to complete its start-up routine. 0 mM of NADH per min at pH 8. 5 and 10 nm were used for excitation and emission, respectively. AB-0558) and immediately read for luminescent signal on a SpectraMax M5 plate reader. 1 or 10 Tablet PC with USB connection to instrument. Quick Guide to the plate reader. 9. Reader Documentation Version No. limit of detection (LLD) for ATP was estimated for each microplate reader using theThe relabeled data may be saved for further analysis as either a . 2. 2 only) SpectraMax M-series Microplate readers. Always use a plate lid and do not place a plate that contains these materials in the plate chamber for prolonged periods of time. Samples were read on a Molecular Devices SpectraMax M5 plate reader at 365-nm excitation and 450-nm emission. Empirical Phase. HSL_EQ_005: Use and Maintenance of a Molecular Devices M5 Plate Reader in the HPV Serology Laboratory 3. 2. Copia stocks a full line of used microplate readers. 5. edu, 814-863-3503. You can use it for many applications (OD, fluorescence, luminescence). The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Inhibition assays at multiple compound. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues are proactively addressed. Dimensions 20" D x 16. Reduce Energy Use by 59% [10] Printing time isn't the only thing getting cut by 70%. Turn the reader on and secure the USB cable. Documents. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86After 5 minutes of incubation at room temperature, luciferase activity was assessed by SpectraMax M5 plate reader (Molecular Probes). 600 in a Molecular Devices SpectraMax M5 plate reader at 30˚C with shaking prior to each reading. Information on cell morphology, localization of signal, cell count, and more is obtained with Cytation 5 imaging mode. SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. Absorbance measured on a Spectra Max M5 plate reader (Molecular Devices). IL-33 concentrations in the extracts were measured with a Quantikine ELISA kit (R&D Systems, Minneapolis, Minn) and read on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, Calif). Fluorescence was measured using microplate reader at an excitation wavelength of 488 nm and emission wavelength of 529 nm using Spectramax-M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). Reactions were performed on a 384-well plate, with release of 6-FAM dye monitored by fluorescence at 517 nm using a SpectraMax M5 plate reader (Molecular Devices) with excitation at 495 nm for 60. Plate chamber design of the SpectraMax category 1 microplate readers. easy to use and read multiple plates very quickly. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Published online 2016 Jul 13. 306 plate air cal fail,low light. 0 out of 5 $ 3,799. Spectranet customer portal GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. Clotting and lysis were monitored in a Spectramax M5 plate reader (Molecular Devices, Berkshire, United Kingdom) at 340nm (plasma) or 405nm (fibrinogen) and analyzed using online apps to determine times to 50% lysis. Fluorescence Microplate Reader Comparison Chart. 025 fmol/well, 384-well plate) Quad monochromator: Fluorescein 2. The Back Panel Figure 1. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Animal modelThe PEG precipitation assay was performed with VRC01-WT mAb at a final protein concentration of 0. Release of free AMC by proteolytic activity was monitored on Molecular Devices M5 plate reader with excitation and emission wavelengths of 360 and 460 nm, respectively. 306 plate air cal fail,low light: 316 grating motor driver faulty: 307 cuv air ref fail: 317 monitor ADC faultyThe BioTek Cytation 5 cell imaging multimode reader combines automated digital widefield microscopy with conventional multimode microplate reading. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. Molecular Devices LLC temperature controlled molecular devices m5 plate reader Temperature Controlled Molecular Devices M5 Plate Reader, supplied by Molecular Devices LLC, used in various techniques. SpectraMax microplate reader with UV absorbance detection (Molecular Devices) UV–transparent microplates Disposable: Corning cat. 0 and room temperature. 1. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. 130 Watts max. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Thermo Scientific™ Nunc MicroWell 96-Well Optical-Bottom Plates with Polymer Base have a solid polystyrene black or white upper structures with a polystyrene film at the bottom of the plate. HSL_EQ_004: Use and Maintenance of a BioTek Plate Washer in the HPV Serology Laboratory 3. The BioTek Cytation cell imaging multimode microplate readers offer modularity to meet today’s workflows and are upgradable to meet future workflow requirements. BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideDetection limit of the plate reader is typically 100–200 RFU, and thus, Eu 3+ has a detection limit of ~100 amol on the SpectraMax M5 plate reader. Viability Assay reagent (Promega). 0200-7027. Kojic acid was used as a positive control. Description Figure 1. , 2018b). 3465 or email us. Imaging opens up a range of applications for cell-based assays that cannot be performed on a standard plate reader. 3) At the bottom of the strip, click on "Show. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. To determine the DAR, STRO-002 in plasma samples were captured using Streptavidin Mag Sepharose Beads (GE Healthcare) coated with biotinylated. Tablet PC. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. 91 cm x 44. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Lastly, Jurkat cells were least susceptible to Vorinostat treatment (all IC 50 > 50. Our comprehensive multipoint inspection and testing ensures each microplate reader or washer meets our product operational specifications. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. In the M2, M5 and Plus 384, the light. 4. This is a result of the light being attenuated and scattered by the. Remove plate cover. Assay was run using JAK1 JH1/JH2 and Omnia Y peptide 12 kit. With optimized reagents, option to operate the. The multi-mode. PMC full text: Oncotarget. The MTS [(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy-After this time, cells were stained with crystal violet, washed 3 times and lysed. 5: Schematic of the back panel of a reader. #3635 or Greiner cat. Clear Filters. Standard curve for Quant-iT OliGreen ssDNA M5 Plate Reader Assay with 200 µl sample volume Quantification of the AAV 8-GFP-CMV viral DNA using the Quant-iT™ OliGreen® ssDNA reagent (Cat. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 2. After the plate is read, data will appear in the plate. Fluorescence Microplate Reader Comparison Chart. CHO-K1 cells were transfected with luciferase. S. Plate chamber design of the SpectraMax category 1 microplate readers. Concentrations of other cytokines were determined by using custom MSD kits and a QuickPlex SQ120 plate reader (Meso Scale Discovery,. Guidelines to using the Qubit Flourimeter. coli cells were purchased from New England Biolabs (Ipswitch, MA). Fluorescence Microplate Readers. harvard. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The guide also covers the software features, the specifications, and the accessories of the systems. siRNA oligonucleotides targeted specifically to rat P2X 7 were used in this experiment. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. Turn on Plate Reader and wait for it to complete its start-up routine. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Varian Inova 500 MHz three-channel Z-axis PFG NMR. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. fluorescence). Preparation of biotin–tyramineThe SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. RNA-Seq . SpectraMax M5 plate reader (Molecular Devices, San Jose, CA) was used. 1. Subsequently, 200 μL of filtrate was transferred into a 96-well UV Star microplate, and the protein concentration in each filtrate was determined using a SpectraMax M5 UV-Visible plate reader. Place the plate in the SpectraMax M5 plate reader immediately, read OD 405 nm every 30s, last 1 h, 37 °C. The samples were loaded into a rectangular quartz cell with the light path length of 1 mm. EPOCH2 Plate Reader/Spectrophotometer. 4. edu, 814-863-3503. Detection modes are available in UV-Vis absorbance, fluorescence, and. Linear, Orbital Slow, and Orbital Fast. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax M4 reader How does the reader perform a self check?. For example, microplates containing different samp les can be read using the same orChoose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. Reader Documentation Version No. Third this recommendation. 5. 18632/oncotarget. Accordingly there are different configurations possible. The fluorescence measurements of cell experiments were conducted with a SpectraMax M5 plate reader. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. The lower . Thermo Scientific Microplate Reader Verification Plates are dedicated performance verification systems helping to verify and document the key performance parameters of Thermo Scientific Multiskan FC, Multiskan Sky, Luminoskan, Fluoroskan FL, and Varioskan LUX. Add 100 μL of this. Reader Documentation Version No. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. 3) At the bottom of the strip, click on "Show. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. The luminescent signal was recorded on a SpectraMax M5 plate reader (Molecular Dy-namics). SpectraMax M-series Microplate readers. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. The fluorometric performance of the SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. The optics of the Gemini XPS Reader are engineered for superior performance and reliability. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Absorbance readings were then taken at 650 nm using a Spectramax M5 plate reader with SoftMax Pro v5. After mixing with freshly diluted substrate (50 μl well −1), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) under luminescence mode. OD 600 and luminescence were measured at 30 minute intervals for 48 hours. Description. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Top reading usually provides better signal-to-noise ratios for solution-based assays such as DNA quantification or protein quantification. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 1% Tween-20 and bound secondary was detected using Ultra TMB (Thermo Fisher); signals were read on a Molecular Devices Spectra Max M5. 3. Cell Titer Glo reagent (Promega) was then added to each well, and luminescence (RLU) was measured on a SpectraMax M5 plate reader (Molecular Devices). Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. † Cells in the paper scaffolds and collected in the well plates were also imaged with an Olympus IX-70 fluorescence microscope equipped with a 10×. Cells were collected and fixed with precooled 95 % ethanol at 4 ºC for 12 h. After addition of the freshly diluted substrate (50 μL/well, from Promega), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices) using the luminescence mode. 500 mm/s is the maximum speed of the extruder of AnkerMake M5 when using Fast Mode. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Louis, MO) dissolved in PBS was injected over 30 seconds into the tail vein of a mouseIn bacteria, natural transposon mobilization can drive adaptive genomic rearrangements. Molecular Devices Spectramax M5 Microplate Reader; Related products. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through. Plate geometry 6- to 384-well plates, Petri dishes Dispense precision ≤2% at 50-200 μL Dispense accuracy ±1 μL or 2% Physical Characteristics Power 250 Watts max. The solids content was measured using a MA35 Moisture Analyzer (Sartorius AG). 0. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Microplate automation and detection instruments for walk away operation and high quality results. Published Dec 16, 2020 | Updated Jun 22, 2023. Patented Hybrid Technology delivers the sensitivity of filters with the flexibility of monochromators. 7. 1. Narrow your selection of used microplate readers using the filters above or select the one of the categories on the left to view multi-mode or. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. High-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities. You can also interpret your results using integrated data analysis software. The measured specific activity of NanoLuc is 1. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. BeWo cells were plated at 1×10 4 cells/well in 96-well opaque walled plates, and viability was assessed at 72H for both CT and SCT cell types as per the manufacturer’s instructions, using a Spectromax M5 plate reader (Molecular Devices, San Jose, CA, USA). Gemini XPS Reader optics. The amount of HRP encapsulated in liposome was calculated from the calibration curve of. 05 were scored as. All enzymes used for DNA manipulations were purchased from New. Supernatant was diluted. After 30 min of induction, cells were transferred to a clear-bottom black 96-well plate, and both GFP fluorescence and optical density were measured using a SpectraMax M5 plate reader. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Wide range of applications. both cuvette-based and microplate reader applications on the same instrument. Cytation 5 extends the legacy of Agilent BioTek multimode plate readers with a modular and upgradable imaging mode. Microplate Reader Shelf. begin reading microplates with seven mouse clicks. MOLECULAR DEVICES VMax Kinetic Microplate Reader For Sale. Reader Documentation Version No. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. The guide also covers the features, specifications, and applications of the reader, as well as troubleshooting tips and technical support information. The WT DENV2C and its single-point mutants L50S, L54S, L81N and I88N were. The threshold of detection was calculated as the mean plus three standard deviations of the luminescence from the supernatant of. Choose the Experiments icon. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Cell apoptosis assay. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. That explains the lower values on the LmaxII384 plate reader. Plates were shaken continuously at medium speed, and temperature was kept at 37°C. Viability was evaluated using the Cell Titer Glo Assay (Promega) and luminescence was read on a SpectraMax M5 plate reader (Molecular Devices). Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. Microplate Reader Shelf. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Step 1: Open the SoftMax Pro IMAP FP protocol. Molecular Devices. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). The Molecular Devices Spectramax M5 Multi-Mode Plate Reader brings you the power and precision of a dedicated single mode plate reader, but with the space saving flexibility of 5 detection modes in one unit. Add 100 μL of sample media and standards (0, 1, 2, 4, 8, 16, 32, 64, 128 μM NaNO 2) to plate in duplicate. We carry both single-mode and multi-mode readers from manufacturers such as BioTek Instruments, Perkin Elmer, and SRU Biosystems. 025 fmol/well, 384-well plate) Quad monochromator: Fluorescein 2. Fluorescence experiments were carried out using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). flexibility of monochromator-based systems. There is currently no charge for using this plate reader. PURPOSE. Softmax Pro GxP Software 6. Signals of wells containing only the target cells. Simply unpack it, plug it in, and begin your experiments. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. 1) Select the correct plate and the wells that you want to measure. The degradation. . Bioluminescence assays showed that the engineered Luc-Cys was fully active when compared with 6 × His-NanoLuc prepared in our previous work (Zhang et al. Transfection of siRNA into cells. Cell growth was monitored by measuring OD values at 600 nm using a SpectraMax M5 plate reader (Molecular Devices, Silicon Valley, CA, United States). GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. 2–200 pg/well). Then, absorbance was measured by a Spectra Max ™ M5 plate reader (Molecular Devices, Sunnyvale, CA). 2016 Aug 2; 7(31): 50027–50042. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. Flock Safety is an Atlanta-based company that sells automatic license plate detection readers, or ALPRs for short, to law enforcement agencies, schools, neighborhood homeowners associations and. I’m. Description Figure 1. S. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. , Sunnyvale, CA, USA) at the excitation wavelength of 488 nm for carboxy-H2DCFDA and 505 nm for DHE and MitoSOX ™. Select the Driver tab. 4 fmol/well, 384-well plate)−bottom Reading Speed (Kinetic) 96 wells: 11 s 384 wells: 22 s Luminescence Wavelength Range 300−700 nm Dynamic Range > 6 decades Sensitivity. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Find regional support. Five preferred detection modes. All values reported represent the mean of at least three independent experiments. The following document is. The password is. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 5: Schematic of the back panel of a reader. 1. The absorbance was read at 450 nm with 650 nm as background correction using Softmax software on M5 plate reader (Molecular Devices). The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. For experiments with TGX-221, the cells were treated with 200 nM of TGX-221 throughout the duration of the experiment.